APPL / DIP13alpha Antibody

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ProSci
Product Code: PSI-43-108
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-108-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-108 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-108 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
APPL1, APPL, adaptor protein containing pH domain, PTB domain and leucine zipper motif, PTB domain and leucine zipper motif 1, DIP13alpha, APPL1, signaling adaptor protein DIP13alpha, AKT2 interactor, adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 90kDa band observed in Human Heart lysates (calculated MW of 79.7kDa according to NP_036228.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR. Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DLGEGGKKRESEA
NCBI Gene ID #:
26060
NCBI Official Name:
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
NCBI Official Symbol:
APPL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_036228.1
Protein GI Number:
6912242
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
Q9UKG1