HNRNPA2B1 Antibody

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ProSci
Product Code: PSI-43-499
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-499-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-499 (0.3ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-499 (0.1ug/ml) staining of NIH3T3 (A), (0.01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-499 (0.3ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-499 (0.1ug/ml) staining of NIH3T3 (A), (0.01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, nuclear ribonucleoprotein particle A2 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Pastor T, Pagani F.Interaction of hnRNPA1/A2 and DAZAP1 with an Alu-derived intronic splicing enhancer regulates ATM aberrant splicing.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DTIEIITDRQS
NCBI Gene ID #:
3181
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein A2/B1
NCBI Official Symbol:
HNRNPA2B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 36kDa
Protein Accession #:
NP_002128.1, NP_112533.1
Protein GI Number:
14043072
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer RNA Binding RNA Binding
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_002128.1; NP_112533.1).
Swissprot #:
P22626