POU6F1 Antibody

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ProSci
Product Code: PSI-43-262
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-262-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-262 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-262 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
POU6F1, POU class 6 homeobox 1, BRN5, MPOU, TCFB1, POU domain, class 6, transcription factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Brain (Cerebral cortex) and Mouse fetal Brain lysates (calculated MW of 32.6kDa according to NP_002693.3). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Pereira JH, Kim SH, Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ETPTVSQLVSKPHTP
NCBI Gene ID #:
5463
NCBI Official Name:
POU class 6 homeobox 1
NCBI Official Symbol:
POU6F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_002693.3
Protein GI Number:
223890225
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience,Transcription,Neuroscience Transcription
Swissprot #:
Q14863