NPHS2 / SRN1 Antibody

Contact us
ProSci
Product Code: PSI-43-360
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-360-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
43-360 (1ug/ml) staining of Human (A) and Mouse (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
❮❯

43-360 (1ug/ml) staining of Human (A) and Mouse (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), PDCN, SRN1, podocin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to Human NP_055440.1 and Mouse NP_569723.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Relle M, Cash H, Brochhausen C, Strand D, Menke J, Galle PR, Schwarting A.New perspectives on the renal slit diaphragm protein podocin.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SPSKPVEPLNPKK
NCBI Gene ID #:
7827
NCBI Official Name:
nephrosis 2, idiopathic, steroid-resistant (podocin)
NCBI Official Symbol:
NPHS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_055440.1
Protein GI Number:
7657615
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction, Stem Cell
Swissprot #:
Q9NP85