tropomyosin 4 / TPM4 Antibody

ProSci
Product Code: PSI-43-548
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-548-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-548 (0.03ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-548 (0.03ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TPM4, tropomyosin 4, TM30p1, tropomyosin alpha-4 chain, tropomyosin-4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 32kDa band observed in Human Platelets, Pig Heart and in NIH3T3 lysates (calculated MW of 32.7kDa according to NP_001138632.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Prostate. Recommended concentration: 2.5ug/ml.
Background References:
  • Vlahovich N, Schevzov G, Nair-Shaliker V, Ilkovski B, Artap ST, Joya JE, Kee AJ, North KN, Gunning PW, Hardeman EC.Tropomyosin 4 defines novel filaments in skeletal muscle associated with muscle remodelling/regeneration in normal and diseased muscle.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ENVGLHQTLDQ
NCBI Gene ID #:
7171
NCBI Official Name:
tropomyosin alpha-4 chain isoform Tpm4.1cy
NCBI Official Symbol:
TPM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 32kDa
Protein Accession #:
NP_001138632.1, NP_003281.1
Protein GI Number:
223555975
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1).
Swissprot #:
P67936