cardiac troponin T Antibody

ProSci
Product Code: PSI-43-671
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-671-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
43-671 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

43-671 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
TNNT2, troponin T type 2 (cardiac), CMH2, CMPD2, LVNC6, MGC3889, RCM3, TnTC, cTnT, OTTHUMP00000033864, OTTHUMP00000033865, OTTHUMP00000033866, OTTHUMP00000033867, OTTHUMP00000033870, OTTHUMP00000218095, cardiac muscle troponin T, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, troponin T, cardiac muscle, troponin T2, cardiac
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human, Mouse and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID: 20083571). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Zhang J, Zhang H, Ayaz-Guner S, Chen YC, Dong X, Xu Q, Ge YPhosphorylation, but not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac troponin T.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TERKSGKRQTERE
NCBI Gene ID #:
7139
NCBI Official Name:
troponin T type 2 (cardiac)
NCBI Official Symbol:
TNNT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 40kDa
Protein Accession #:
NP_000355.2, NP_001001430.1, NP_001001431.1, NP_001001432.1
Protein GI Number:
21264536
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1).
Swissprot #:
P45379