ACAT1 Antibody

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ProSci
Product Code: PSI-43-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-137-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-137 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-137 (0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1 and 44.81kDa to Mouse NP_659033.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa. This data is from a previous batch, not on sale.
Background References:
  • Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DEEYKRVDFSKVPK
NCBI Gene ID #:
38
NCBI Official Name:
acetyl-CoA acetyltransferase 1
NCBI Official Symbol:
ACAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_000010.1
Protein GI Number:
4557237
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Signal Transduction,Cancer Signal Transduction
Swissprot #:
P24752