calbindin D28 Antibody
| Code | Size | Price |
|---|
| PSI-43-170-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CALB1, calbindin 1, 28kDa, CALB, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, calbindin, calbindin 1, (28kD), calbindin D28, vitamin D-dependent calcium-binding protein, avian-type
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
- Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y.DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KTFVDQYGQRDDGK
NCBI Gene ID #:
793
NCBI Official Name:
calbindin 1, 28kDa
NCBI Official Symbol:
CALB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 26kDa
Protein Accession #:
NP_004920.1
Protein GI Number:
115394
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Obesity,
SPECIFICITY:
Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Swissprot #:
P05937