contactin 1 Antibody

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ProSci
Product Code: PSI-43-466
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-466-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-466 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-466 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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43-466 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-466 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
CNTN1, contactin 1, F3, GP135, contactin-1, glycoprotein gP135, neural cell surface protein F3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in Mouse Brain, and approx 150kDa observed in Human and Rat Brain lysates (calculated MW of 113kDa according to according to Human, NP_001834.2, Mouse NP_031753.1 and Rat NP_476459.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Mouse Brain (Cerebral Cortex). Recommended concentration: 5ug/ml.
Background References:
  • Lamprianou S, Chatzopoulou E, Thomas JL, Bouyain S, Harroch S.A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-QVTSQEYSARLEN
NCBI Gene ID #:
1272
NCBI Official Name:
contactin 1
NCBI Official Symbol:
CNTN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 150kDa
Protein Accession #:
NP_001834.2, NP_778203.1
Protein GI Number:
28373117
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Stem Cell,
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_001834.2) and isoform 2 (NP_778203.1).
Swissprot #:
Q12860