GFI1b Antibody
| Code | Size | Price |
|---|
| PSI-79-763-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
BDPLT17, Gfi-1b
Application Note:
Western Blot: 1:500~1:1000
Background:
Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
Background References:
- Osawa M., Yamaguchi T., Nakamura Y., Kaneko S., Onodera M., Sawada K., Jegalian A., Wu H., Nakauchi H., Iwama A. Blood 100:2769-2777 (2002) Huang D. -Y., Kuo Y. -Y., Chang Z. -F. Nucleic Acids Res. 33:5331-5342 (2005) Vassen L., Fiolka K., Moeroey T. EMBO J. 25:2409-2419 (2006)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GFI1b antibody? was raised against a peptide sequence around aa.322~326 (H-R-E-S-Q) derived from Human GFI1b.
NCBI Gene ID #:
8328
NCBI Official Name:
growth factor independent 1B transcription repressor
NCBI Official Symbol:
GFI1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
NP_001128503.1
Protein GI Number:
205277345
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Stem Cell
SPECIFICITY:
This antibody detects endogenous level of total GFI1b protein.
Swissprot #:
Q5VTD9