ACTN1 Antibody
| Code | Size | Price |
|---|
| PSI-43-554-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ACTN1, actinin, alpha 1, F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103kDa according to NP_001093.1). Recommended concentration: 0.3-1ug/ml.
An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Quick Q, Skalli O.Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TPQEINGKWDHVRQ
NCBI Gene ID #:
87
NCBI Official Name:
actinin, alpha 1
NCBI Official Symbol:
ACTN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_001123476.1, NP_001093.1, NP_001123477.1
Protein GI Number:
4501891
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001123476.1; NP_001093.1; NP_001123477.1).
Swissprot #:
P12814