SERCA2 / ATP2A2 Antibody

ProSci
Product Code: PSI-43-428
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-428-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-428 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-428 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ATP2A2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, ATP2B, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, MGC45367, SERCA2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, SR Ca(2+)-ATPase 2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kDa according to NP_001672.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Background References:
  • Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K.Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: DELNPSAQRDACLN
NCBI Gene ID #:
488
NCBI Official Name:
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
NCBI Official Symbol:
ATP2A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 110kDa
Protein Accession #:
NP_001672.1, NP_733765.1
Protein GI Number:
4502285
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Obesity,
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Swissprot #:
P16615