TNNT3 Antibody

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ProSci
Product Code: PSI-43-294
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-294-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-294 (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-294 (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
TNNT3, troponin T type 3 (skeletal, fast), TNTF, beta TnTF, beta-TnTF, fTnT, fast skeletal muscle troponin T, troponin T, fast skeletal muscle, troponin-T3, skeletal, fast
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8ug/ml.
Background References:
  • Zhao N, Jiang M, Han W, Bian C, Li X, Huang F, Kong Q, Li J.A novel mutation in TNNT3 associated with Sheldon-Hall syndrome in a Chinese family with vertical talus.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TTLRSRIDQAQKHSK
NCBI Gene ID #:
7140
NCBI Official Name:
troponin T type 3 (skeletal, fast)
NCBI Official Symbol:
TNNT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 35kDa
Protein Accession #:
NP_006748.1, NP_001036246.1, NP_001036247.1
Protein GI Number:
112789536
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). Reported variants represent identical protein: NP_001284575.1, NP_001036247.1
Swissprot #:
P45378