TNNI3 Antibody
| Code | Size | Price |
|---|
| PSI-43-244-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Porcine (pig)
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TNNI3, troponin I type 3 (cardiac), CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25+26kDa doublet band observed in Human Heart, approx 24+27kDa in Mouse Heart, approx 25kDa in Rat Heart and approx 27kDa in Pig Heart lysates (calculated MW of 24kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3kDa according to Mouse NP_033432.1 and Ra tNP_058840.1 ). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Basta G, Del Turco S, Marchi F, Navarra T, Battaglia D, Mercuri A, Mazzone A, Berti S.Elevated soluble receptor for advanced glycation end product levels in patients with acute coronary syndrome and positive cardiac troponin I.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KVTKNITEIAD
NCBI Gene ID #:
7137
NCBI Official Name:
troponin I type 3 (cardiac)
NCBI Official Symbol:
TNNI3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 27 + 28kDa
Protein Accession #:
NP_000354.4
Protein GI Number:
136213
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Stem Cell,Monoclonal,Monoclonal Signal Transduction Stem Cell
Swissprot #:
P19429