CAPG Antibody

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ProSci
Product Code: PSI-43-521
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-521-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-521 (0.3ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-521 (0.3ug/ml) staining of Pig Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-521 (0.3ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-521 (0.3ug/ml) staining of Pig Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CAPG, capping protein (actin filament), gelsolin-like, AFCP, MCP, actin regulatory protein CAP-G, actin-regulatory protein CAP-G, gelsolin-like capping protein, macrophage capping protein, macrophage-capping protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Hubert T, Van Impe K, Vandekerckhove J, Gettemans J.The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KAQVEIVTDGEEP
NCBI Gene ID #:
822
NCBI Official Name:
capping protein (actin filament), gelsolin-like
NCBI Official Symbol:
CAPG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 38kDa
Protein Accession #:
NP_001738.2
Protein GI Number:
4502561
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_001738.2) only. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
Swissprot #:
P40121