PCNA Antibody
| Code | Size | Price |
|---|
| PSI-43-158-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Porcine (pig)
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa according to Mouse NP_035175.1, Rat NP_071776.1 and Pig NP_001278854.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.01-0.03ug/ml.Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB11651.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Jones MJ, Colnaghi L, Huang TT.Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NGNIKLSQTSNVD
NCBI Gene ID #:
5111
NCBI Official Name:
proliferating cell nuclear antigen
NCBI Official Symbol:
PCNA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_002583.1
Protein GI Number:
4505641
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Transcription,Cancer Transcription
SPECIFICITY:
Reported variants represent identical protein: NP_872590.1, NP_002583.1
Swissprot #:
P12004