LDHB Antibody

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ProSci
Product Code: PSI-43-546
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-546-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-546 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-546 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6kDa according to NP_002291.1). Recommended concentration: 0.03-0.1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Kim JH, Kim EL, Lee YK, Park CB, Kim BW, Wang HJ, Yoon CH, Lee SJ, Yoon G.Decreased lactate dehydrogenase B expression enhances claudin 1-mediated hepatoma cell invasiveness via mitochondrial defects.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NARGLTSVINQKLK
NCBI Gene ID #:
3945
NCBI Official Name:
lactate dehydrogenase B
NCBI Official Symbol:
LDHB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 35kDa
Protein Accession #:
NP_002291.1
Protein GI Number:
4557032
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Signal Transduction,Transcription,
SPECIFICITY:
Reported variants represent identical protein: NP_002291.1, NP_001167568.1.
Swissprot #:
P07195