PRMT2 Antibody

ProSci
Product Code: PSI-43-163
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-163-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-163 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-163 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PRMT2, protein arginine methyltransferase 2, HRMT1L1, MGC111373, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1, HMT1 hnRNP methyltransferase-like 1, OTTHUMP00000046525, OTTHUMP00000046526, OTTHUMP00000115881, OTTHUMP00000115882, OTTHUMP00000115884, OTTHUMP00000207831, PRMT2 alpha, PRMT2 beta, PRMT2 gamma, histone-arginine N-methyltransferase PRMT2, protein arginine N-methyltransferase 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line HeLa and in rodent Brain lysates (calculated MW of 37.2kDa according to NP_001229793.1). Approx 35kDa band observed in Pig Brain lysates (calculated MW of 38.7kDa according to XP_005658963.1).Recommended concentration: 1-3ug/ml.
Background References:
  • Pak ML, Lakowski TM, Thomas D, Vhuiyan MI, H?secken K, Frankel A.A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DQPRTTKYHSVILQ
NCBI Gene ID #:
3275
NCBI Official Name:
protein arginine methyltransferase 2
NCBI Official Symbol:
PRMT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 37kDa
Protein Accession #:
NP_001526.2, NP_001229793.1, NP_001229794.1, NP_001229795.1
Protein GI Number:
46255047
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1). Reported variants represent identical protein: NP_001526.2, NP_996845.1
Swissprot #:
P55345