Vinculin Antibody (biotin)
Code | Size | Price |
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PSI-7807-biotin-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-7807-biotin-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Human
- Mouse
- Rabbit
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Biotin-Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
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Further Information
Additional Names:
CMD1W, CMH15, Metavinculin, MVCL
Application Note:
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background References:
- Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
- Gilmore et al. J. Cell Sci. 1992; 103:719-31.
- Deakin et al. PLoS One 2012; 7:e37990.
- Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Buffer:
Biotin-Vinculin Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
NCBI Gene ID #:
7414
NCBI Official Name:
vinculin
NCBI Official Symbol:
VCL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 117 kDa
Observed: 117 kDa
Observed: 117 kDa
Protein Accession #:
NP_003364
Protein GI Number:
4507877
Purification:
Biotin-Vinculin antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
Biotin-Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Swissprot #:
P18206
User NOte:
Optimal dilutions for each application to be determined by the researcher.