GAPDH Antibody (biotin)

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ProSci
Product Code: PSI-3781-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3781-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-3781-biotin-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Biotin-GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, mouse lung, mouse liver, rat liver, and chicken small intestine lysate with Biotin-GAPDH antibody at 1 μg/mL.
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Western blot analysis of GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, mouse lung, mouse liver, rat liver, and chicken small intestine lysate with Biotin-GAPDH antibody at 1 μg/mL.

Documents

Further Information

Additional Names:
Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Application Note:
Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer?s and Huntington?s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
  • Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
  • Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
Buffer:
Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
NCBI Gene ID #:
2597
NCBI Official Name:
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 37 kDa

Observed: 36 kDa
Protein Accession #:
NP_002037
Protein GI Number:
7669492
Purification:
Biotin-GAPDH antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience,Homeostasis
SPECIFICITY:
Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Swissprot #:
P04406
User NOte:
Optimal dilutions for each application to be determined by the researcher.