Alpha-tubulin Antibody (biotin)

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ProSci
Product Code: PSI-7597-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7597-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-7597-biotin-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rabbit
  • Rat
  • Zebrafish
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Biotin-Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of Alpha-tubulin in multiple cell and tissue lysates with Biotin-Alpha-tubulin antibody at 1 μg/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, chicken small intestine, and zebrafish lysate, respectively.
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Western blot analysis of Alpha-tubulin in multiple cell and tissue lysates with Biotin-Alpha-tubulin antibody at 1 μg/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, chicken small intestine, and zebrafish lysate, respectively.

Documents

Further Information

Additional Names:
Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
Application Note:
Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
Background References:
  • McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
  • Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
  • Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.
Buffer:
Biotin-Alpha-tubulin antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-Alpha-tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-tubulin
NCBI Gene ID #:
7846
NCBI Official Name:
tubulin, alpha 1a
NCBI Official Symbol:
TUBA1A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa

Observed: 55 kDa
Protein Accession #:
CAA25855
Protein GI Number:
37492
Purification:
Biotin-Alpha-tubulin antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
Biotin-Alpha-tubulin antibody is human, mouse, rat, rabbit, chicken, and zebrafish reactive.
Swissprot #:
Q71U36
User NOte:
Optimal dilutions for each application to be determined by the researcher.