PRKCB Antibody

ProSci
Product Code: PSI-43-319
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-319-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-319 (0.3ug/ml) staining of Human Placenta (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-319 (0.3ug/ml) staining of Human Placenta (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Sheng YJ, Gao JP, Li J, Han JW, Xu Q, Hu WL, Pan TM, Cheng YL, Yu ZY, Ni C, Yao S, He CF, Liu YS, Li Y, Ge HM, Xiao FL, Sun LD, Yang S, Zhang XJ.Follow-up study identifies two novel susceptibility loci PRKCB and 8p11.21 for systemic lupus erythematosus.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DKEFTRQPVELT
NCBI Gene ID #:
5579
NCBI Official Name:
protein kinase C, beta
NCBI Official Symbol:
PRKCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 80kDa
Protein Accession #:
NP_997700.1
Protein GI Number:
20127450
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Cancer,Neuroscience,Apoptosis Cancer Neuroscience
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_997700.1) only.
Swissprot #:
P05771