ZNRF1 Antibody

Contact us
ProSci
Product Code: PSI-43-275
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-275-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 3
N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with 43-275 (1ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 3
3 / 3
❮❯

N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with 43-275 (1ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
Znrf1, zinc and ring finger 1, B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283, E3 ubiquitin-protein ligase ZNRF1, nerve injury-induced gene 283 protein, peripheral nerve injury protein nin283, ring finger protein 42, zinc ring finger protein 1, zinc/RING finger protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, JapanImmunohistochemistry:In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SDSTYAHGNGYQET
NCBI Gene ID #:
84937
NCBI Official Name:
zinc and ring finger 1, E3 ubiquitin protein ligase
NCBI Official Symbol:
Znrf1
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 28kDa
Protein Accession #:
NP_573469.1, NP_001162093.1
Protein GI Number:
18875354
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1
Swissprot #:
Q8ND25