Nalp12 / Nlrp12 Antibody

ProSci
Product Code: PSI-43-426
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-426-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-426 (1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-426 (1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Nlrp12, NLR family, pyrin domain containing 12, Nalp12, PYPAF7, NACHT, LRR and PYD containing protein 12, NACHT, LRR and PYD domains-containing protein 12, PYRIN-containing APAF1-like protein 7, monarch-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse Liver and Spleen lysates (calculated MW of 119kDa according to NP_001028603.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (J?ru et al, Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1614-9.PMID: 18230725). Recommended concentration: 1-3ug/ml.
Background References:
  • Allen IC, Wilson JE, Schneider M, Lich JD, Roberts RA, Arthur JC, Woodford RM, Davis BK, Uronis JM, Herfarth HH, Jobin C, Rogers AB, Ting JP.NLRP12 suppresses colon inflammation and tumorigenesis through the negative regulation of noncanonical NF-κB signaling.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-KPGTPTFKVPANQR
NCBI Gene ID #:
378425
NCBI Official Name:
NLR family, pyrin domain containing 12
NCBI Official Symbol:
Nlrp12
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_001028603.1
Protein GI Number:
257468329
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Growth Factors,Neuroscience,Signal Transduction,Stem Cell,
Swissprot #:
E9Q5R7