MEX3C Antibody

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ProSci
Product Code: PSI-43-154
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-154-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-154 (0.2ug/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35ug protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Detected by chemiluminescence.
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43-154 (0.2ug/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35ug protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MEX3C, mex-3 homolog C (C. elegans), FLJ38871, MEX-3C, RKHD2, RNF194, OTTHUMP00000163545, RING finger and KH domain-containing protein 2, RING finger protein 194, RNA-binding protein MEX3C, ring finger and KH domain containing 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60+36kDa bands observed in Mouse Testis lysates, and these bands reduced after knock-down by gene trap (calculated MW of 69.4kDa according to NP_057710.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Buchet-Poyau K, Courchet J, Le Hir H, S?raphin B, Scoazec JY, Duret L, Domon-Dell C, Freund JN, Billaud M.Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA binding proteins differentially localized to processing bodies.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TPRLSPTFPESIEH
NCBI Gene ID #:
51320
NCBI Official Name:
mex-3 homolog C (C. elegans)
NCBI Official Symbol:
MEX3C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60 + 36kDa
Protein Accession #:
NP_057710.3
Protein GI Number:
148229134
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Apoptosis
Swissprot #:
Q5U5Q3