SIRT1 Antibody
| Code | Size | Price |
|---|
| PSI-43-119-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SIRT1 , sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , RP11-57G10.3, SIR2L1 , OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 80.3kDa according to Mouse NP_062786.1). Preliminary testing also showed a band at approx 120xkDa in Fetal Mouse Brain lysate. The observed molecular weights correspond to different antibodies in the literature and from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Background References:
- Chen D, Steele AD, Lindquist S, Guarente L. Increase in activity during calorie restriction requires Sirt1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EITEKPPRTQKE
NCBI Gene ID #:
23411
NCBI Official Name:
sirtuin 1
NCBI Official Symbol:
SIRT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 120kDa
Protein Accession #:
NP_036370.2
Protein GI Number:
7657575
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
Swissprot #:
Q96EB6