ACHRE / CHRNE Antibody

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ProSci
Product Code: PSI-43-230
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-230-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-230 (1ug/ml) staining of Mouse Fetal Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-230 (1ug/ml) staining of Mouse Fetal Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CHRNE, cholinergic receptor, nicotinic, epsilon, ACHRE, CMS1D, CMS1E, CMS2A, FCCMS, SCCMS, AchR epsilon subunit, cholinergic receptor, nicotinic, epsilon polypeptide, nicotinic acetylcholine receptor epsilon polypeptide
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 55kDa band observed in Mouse Fetal Skeletal Muscle lysates (calculated MW of 54.9kDa according to NP_033733.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Faber CG, Molenaar PC, Vles JS, Bonifati DM, Verschuuren JJ, van Doorn PA, Kuks JB, Wokke JH, Beeson D, De Baets M, AChR deficiency due to epsilon-subunit mutations: two common mutations in the Netherlands.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-DQEATGEEVSD
NCBI Gene ID #:
1145
NCBI Official Name:
cholinergic receptor, nicotinic, epsilon (muscle)
NCBI Official Symbol:
CHRNE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 55kDa
Protein Accession #:
NP_000071.1
Protein GI Number:
4557463
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction Membrane Membrane
Swissprot #:
Q04844