Nanog Antibody

Contact us
ProSci
Product Code: PSI-43-623
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-623-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
43-623 (0.5ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
❮❯

43-623 (0.5ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
Nanog, Nanog homeobox, 2410002E02Rik, ENK, ecat4, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox protein NANOG, homeobox transcription factor Nanog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 34.6kDa according to NP_082292.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zbinden et al, EMBO J. 2010 Aug 4;29(15):2659-74. PMID: 20581802). Recommended concentration: 0.5-2ug/ml.
Background References:
  • Han J, Mistriotis P, Lei P, Wang D, Liu S, Andreadis ST.Nanog reverses the effects of organismal aging on mesenchymal stem cell proliferation and myogenic differentiation potential.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DLEVNLEATRESHAH
NCBI Gene ID #:
71950
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
Nanog
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 40kDa
Protein Accession #:
NP_082292.1
Protein GI Number:
110625918
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Signal Transduction,
Swissprot #:
Q5TM83