JUND Antibody

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ProSci
Product Code: PSI-43-498
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-498-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-498 (1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-498 (1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
JUND, jun D proto-oncogene , JunD-FL isoform, transcription factor jun-D
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 35.2kDa according to NP_005345.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Short and Pfarr,J Biol Chem. 2002 Sep 6;277(36):32697-705. PMID: 12105216). Recommended concentration: 1-3ug/ml.
Background References:
  • Hartman TR, Qian S, Bolinger C, Fernandez S, Schoenberg DR, Boris-Lawrie K. RNA helicase A is necessary for translation of selected messenger RNAs.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: CQLLPQHQVPAY
NCBI Gene ID #:
3727
NCBI Official Name:
jun D proto-oncogene
NCBI Official Symbol:
JUND
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 40kDa
Protein Accession #:
NP_005345.3
Protein GI Number:
102469037
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Transcription,Cancer Transcription
SPECIFICITY:
This antibody is expected NOT to cross-react with Jun oncogen (NP_002219.1, GeneID 3725)
Swissprot #:
P17535