Crot Antibody

ProSci
Product Code: PSI-43-306
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-306-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 3
43-306 (1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 3
3 / 3

43-306 (1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
Crot, carnitine O-octanoyltransferase, COT, peroxisomal carnitine O-octanoyltransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Mouse Liver lysates (calculated MW of 70.3kDa according to NP_114193.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5ug/ml.
Background References:
  • Li YL, Vergne J, Torchet C, Maurel MC, In vitro selection of adenine-dependent ribozyme against Tpl2/Cot oncogene.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: NYFKTESEGHCPTH
NCBI Gene ID #:
83842
NCBI Official Name:
carnitine O-octanoyltransferase
NCBI Official Symbol:
Crot
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_114193.1
Protein GI Number:
14010893
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction, Stem Cell
Swissprot #:
P11466