betaCstF-64 variant 1/ betaCstF-64 variant 3 Antibody

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ProSci
Product Code: PSI-43-424
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-424-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ?Cstf-64 (DYKDDDDK tagged) immunoprecipitated using 43-424 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDD
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HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ?Cstf-64 (DYKDDDDK tagged) immunoprecipitated using 43-424 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDD

Documents

Further Information

Additional Names:
Cstf2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64kDa, C630034J23Rik, Cstf64, CF-1 64 kDa subunit, CSTF 64 kDa subunit, alphaCstF-64 variant 4, betaCstF-64 variant 1, betaCstF-64 variant 3, cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa, cstF-64
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Immunoprecipitation: In transfected HeLa transiently expressing Mouse ?Cstf-64 ((DYKDDDDK tagged), a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2ug per lysate. Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed ?Cstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both ?Cstf-64 anf Cstf-64 Recommended amount 2ug EB11838/500ug lysate protein.
Background References:
  • Shankarling GS, Coates PW, Dass B, Macdonald CC.A family of splice variants of CstF-64 expressed in vertebrate nervous systems.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PASIERVQGQRT
NCBI Gene ID #:
108062
NCBI Official Name:
cleavage stimulation factor, 3' pre-RNA subunit 2
NCBI Official Symbol:
Cstf2
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
ACF05702.1, ACF05700.1
Protein GI Number:
192764316
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Stem Cell
SPECIFICITY:
This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
Swissprot #:
Q8BIQ5