Myosin IH Antibody

ProSci
Product Code: PSI-43-355
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-355-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-355 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-355 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventional myosin-Ih
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Tassopoulou-Fishell M, Deeley K, Harvey EM, Sciote J, Vieira AR.Genetic variation in myosin 1H contributes to mandibular prognathism.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-EDQVYKDKNGQLR
NCBI Gene ID #:
283446
NCBI Official Name:
myosin IH
NCBI Official Symbol:
Myo1H
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 110kDa
Protein Accession #:
NP_001158045.1
Protein GI Number:
257196127
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Neuroscience,Obesity,Stem Cell,
Swissprot #:
Q8N1T3