Pycard Antibody

ProSci
Product Code: PSI-43-303
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-303-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-303 (0.5ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

43-303 (0.5ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

Further Information

Additional Names:
Pycard, PYD and CARD domain containing, 9130417A21Rik, Asc, CARD5, TMS-1, TNS1, masc, PYD and CARD domain-containing protein, apoptosis-associated speck-like protein containing a CARD
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 21+24kDa band observed in Mouse Spleen lysates (calculated MW of 21.5kDa according to NP_075747.3). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Compan V, Baroja-Mazo A, L?pez-Castej?n G, Gomez AI, Mart?nez CM, Angosto D, Montero MT, Herranz AS, Baz?n E, Reimers D, Mulero V, Pelegr?n P.Cell Volume Regulation Modulates NLRP3 Inflammasome Activation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DLTDKLVSYYLES
NCBI Gene ID #:
29108
NCBI Official Name:
PYD and CARD domain containing
NCBI Official Symbol:
Pycard
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 21 + 23kDa
Protein Accession #:
NP_075747.3
Protein GI Number:
10801602
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Apoptosis
Swissprot #:
Q9ULZ3