CLOCK Antibody

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ProSci
Product Code: PSI-43-364
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-364-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-364 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-364 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CLOCK, clock homolog (mouse), KAT13D, bHLHe8, circadian locomoter output cycles kaput protein, circadian locomoter output cycles protein kaput, class E basic helix-loop-helix protein 8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.4kDa according to Mouse NP_031741.1 and 97.0kDa according to Rat NP_068628.1). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Williams SR, Zies D, Mullegama SV, Grotewiel MS, Elsea SH.Smith-Magenis syndrome results in disruption of CLOCK gene transcription and reveals an integral role for RAI1 in the maintenance of circadian rhythmicity.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Cow
Immunogen:
The immunogen for this antibody is: C-DTSTPPRQHLPAHEK
NCBI Gene ID #:
9575
NCBI Official Name:
clock circadian regulator
NCBI Official Symbol:
CLOCK
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_004889.1
Protein GI Number:
4758010
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Obesity
SPECIFICITY:
Reported variants represent identical protein: NP_004889.1, NP_001254772.1
Swissprot #:
O15516