ADAM12 Antibody

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ProSci
Product Code: PSI-43-502
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-502-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-502 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-502 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ADAM12, ADAM metallopeptidase domain 12, MCMP, MCMPMltna, MLTN, MLTNA, disintegrin and metalloproteinase domain-containing protein 12, meltrin-alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Heart lysates and approx 90+60kDa bands observed in Mouse, Rat and Pig Heart lysates (calculated MW of 99.5kDa according to NP_003465.3 and of 80.4kDa according to NP_067673.2). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Ray BK, Dhar S, Shakya A, Ray A.Z-DNA-forming silencer in the first exon regulates human ADAM-12 gene expression.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-REFQRQGKDLEKVKQ
NCBI Gene ID #:
8038
NCBI Official Name:
ADAM metallopeptidase domain 12
NCBI Official Symbol:
ADAM12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90 + 60kDa
Protein Accession #:
NP_003465.3, NP_067673.2
Protein GI Number:
73747885
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).
Swissprot #:
O43184