Peripherin 1 Antibody

ProSci
Product Code: PSI-43-544
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-544-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-544 (0.1ug/ml) staining of Rat Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-544 (0.1ug/ml) staining of Rat Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Prph, peripherin, Prph1, peripherin 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Rat Colon lysates (calculated MW of 54.0kDa according to NP_036765.2). Recommended concentration: 0.1-0.3ug/ml. An additional faint band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide.
Background References:
  • McLean JR, Robertson J.Isoform-specific expression and ratio changes accompany oxidant-induced peripherin aggregation in a neuroblastoma cell line.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-QKEQHSDLDKSSIH
NCBI Gene ID #:
19132
NCBI Official Name:
peripherin
NCBI Official Symbol:
Prph
NCBI Organism:
Mus musculus?
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 55kDa
Protein Accession #:
NP_038667.2, NP_001157060.1, NP_001157061.1
Protein GI Number:
254675335
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1).
Swissprot #:
P15331