Hsp31p Antibody

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ProSci
Product Code: PSI-43-196
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-196-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Saccharomyces cerevisiae
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-196 (0.05ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny
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43-196 (0.05ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny

Documents

Further Information

Additional Names:
Hsp31p, YDR533C
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1). Recommended concentration: 0.02-0.06ug/ml. This antibody has been successfully used in the following paper: Natka?ska et al (2018) PMID: 29264711.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ETGKFGWDEHSLAKD
NCBI Gene ID #:
852146
NCBI Official Name:
Hsp31p
NCBI Official Symbol:
Hsp31p
NCBI Organism:
Saccharomyces cerevisiae
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 24kDa
Protein Accession #:
NP_010822.1
Protein GI Number:
6320742
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
Q04432