NELL2 Antibody

ProSci
Product Code: PSI-43-562
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-562-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-562 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

43-562 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
NELL2, NEL-like 2 (chicken), NRP2, NEL-like protein 2, NEL-related protein 2, neural epidermal growth factor-like 2, protein kinase C-binding protein NELL2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Brain (cortex and hippocampus) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.7kDa for the mature form (aa22-866) according to NP_001138579.1 and 88.8kDa (aa22-816) according to NP_001138580.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Background References:
  • Kahle JJ, Gulbahce N, Shaw CA, Lim J, Hill DE, Barab?si AL, Zoghbi HY.Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DPCQADTIRNDITKT
NCBI Gene ID #:
4753
NCBI Official Name:
NEL-like 2 (chicken)
NCBI Official Symbol:
NELL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001138579.1, NP_006150.1, NP_001138581.1, NP_001138582.1
Protein GI Number:
5453766
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction Membrane Membrane
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1). Reported variants represent identical protein: NP_001138580.1, NP_006150.1.
Swissprot #:
Q99435