MED13L Antibody

ProSci
Product Code: PSI-43-458
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-458-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
MED13L, mediator complex subunit 13-like, PROSIT240, THRAP2, TRAP240L, mediator of RNA polymerase II transcription subunit 13-like, thyroid hormone receptor-associated protein 2, thyroid hormone receptor-associated protein complex 240 kDa component-like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 80kDa band in lysates of cell line HeLa after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_056150.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Muncke N, Jung C, R?diger H, Ulmer H, Roeth R, Hubert A, Goldmuntz E, Driscoll D, Goodship J, Sch?n K, Rappold G.Missense mutations and gene interruption in PROSIT240, a novel TRAP240-like gene, in patients with congenital heart defect (transposition of the great arteries).
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SPKRSGKIPPKLHNH
NCBI Gene ID #:
23389
NCBI Official Name:
mediator complex subunit 13-like
NCBI Official Symbol:
MED13L
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
Protein Accession #:
NP_056150.1
Protein GI Number:
44771211
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
Q71F56