RAP80 Antibody

ProSci
Product Code: PSI-43-132
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-132-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-132 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

43-132 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
UIMC1, ubiquitin interaction motif containing 1, RAP80, X2HRIP110, OTTHUMP00000161441, receptor associated protein 80, retinoid x receptor interacting protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7kDa according to NP_057374.3. The 60kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Background References:
  • Nikkil? J, Coleman KA, Morrissey D, Pylk?s K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA.Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-TRRQKEAKTKSDSG
NCBI Gene ID #:
51720
NCBI Official Name:
ubiquitin interaction motif containing 1
NCBI Official Symbol:
UIMC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057374.3
Protein GI Number:
119605449
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
Swissprot #:
Q96RL1