DAF-FM Solution
| Code | Size | Price |
|---|
| CDX-A0523-M001 | 1 mg | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
Diaminofluorescein-FM; 4-Amino-5-methylamino-2',7'-difluorofluorescein
Appearance:
Clear colourless to yellow liquid.
CAS:
254109-20-1
Class:
3
Concentration:
5mM in DMSO (1mg in 0.49ml DMSO).
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
InChiKey:
BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 254109-20-1. Formula: C21H14F2N2O5. MW: 412.34. Synthetic. DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm and a dectection limit of 3 nM, DAF-FM can be detected using any instrument that can be used to detect fluorescein. It is more photo-stable and less pH sensitive than DAF-2.
MDL:
MFCD31010374
Molecular Formula:
C21H14F2N2O5
Molecular Weight:
412.34
Package Type:
Vial
PG:
III
Product Description:
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm and a dectection limit of 3 nM, DAF-FM can be detected using any instrument that can be used to detect fluorescein. It is more photo-stable and less pH sensitive than DAF-2.
Purity:
>98% (HPLC)
SMILES:
CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Excepted Quantity
UN Nummer:
UN 1993
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)