Mouse anti Lamin A and C conjugated to FITC
| Code | Size | Price |
|---|
| MUB1102L1 | 1ml | £406.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 131C3
Regulatory Status: RUO
Application: Flow Cytometry
Storage:
Store at 40 or in small aliquots at -200.
Documents
Further Information
Applications Description:
131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry.
Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.
Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.
Background:
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Field of Interest:
Cytoskeleton|Neuroscience|Cell Biology
Formulation:
Each vial contains 1ml FITC-conjugated anti lamin A and C monoclonal antibody in PBS containing 0.1% BSA, 0.09% sodium azide. Approximately 100 tests.
Label:
FITC
Product:
Each vial contains 1ml FITC-conjugated anti lamin A and C monoclonal antibody in PBS containing 0.1% BSA, 0.09% sodium azide. Approximately 100 tests.
Product Form:
FITC
Source:
131C3 is a mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.
Specificity:
131C3 reacts with an epitope located between residues 319-566 in lamin A and C.
UniProt:
P02545
References
1. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110:2483-93.
2. Neri, L. M., Raymond, Y., Giordano, A., Borgatti, P., Marchisio, M., Capitani, S., and Martelli, A. M. (1999). Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions, J Cell Biochem 75:36-45.
3. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178:284-95.
2. Neri, L. M., Raymond, Y., Giordano, A., Borgatti, P., Marchisio, M., Capitani, S., and Martelli, A. M. (1999). Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions, J Cell Biochem 75:36-45.
3. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178:284-95.