VASP Antibody [IE273]

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ProSci
Product Code: PSI-36-170
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-170-50ug50ug£657.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: IE273
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Porcine (pig)
  • Rabbit
  • Sheep
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Vasodilator-stimulated Phosphoprotein
Application Note:
Immunocytochemistry: (1-5ug/ml, 1:100-1:500) . Western Blot: (0.01-0.25ug/ml, 1:2000-1:50,000). Optimal conditions must be determined individually for each application.
Background:
VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
Buffer:
Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide.
Concentration:
500 ug/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified human VASP.
NCBI Gene ID #:
7408
NCBI Official Name:
vasodilator-stimulated phosphoprotein
NCBI Official Symbol:
VASP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
4507869
Protein GI Number:
NP_003361.1
Purification:
Protein A/G purified.
Research Area:
Cancer,Immunology,Innate Immunity
SPECIFICITY:
Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human, porcine, bovine, sheep and rabbit VASP.
Swissprot #:
P50552
User NOte:
Optimal dilutions for each application to be determined by the researcher.