BimS/EL/L Antibody [3C5]

BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death

ChIP: (1:200) Band shift assay: Competes with probe for NFAT binding. . Immunoprecipitation: (1:200) . Western Blot: (1:500-1:1000) . Optimal conditions must be determined individually for each application.

Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.

Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.

1 mg/ml

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Synthetic peptide corresponding to aa 20-40 of mouse BimL.

Liquid

>95% (SDS-PAGE)

Apoptosis,Cancer

Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.

Optimal dilutions for each application to be determined by the researcher.