Caspase-2 Antibody [10C6]

CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2

Flow Cytometry: (overexpressed in cell lines) . Immunocytochemistry: (10-20ug/ml; fix in 4% paraformaldehyde, 0.18% Triton-X100 for 10 min. prior to staining) . Immunohistochemistry: (frozen sections). Optimal conditions must be determined individually for each application.

Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

Liquid. In PBS containing 0.02% sodium azide.

1 mg/ml

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Recombinant human caspase-2 (p19 subunit).

835

caspase 2, apoptosis-related cysteine peptidase

CASP2

Homo sapiens

Liquid

83300977

P42575

>95% (SDS-PAGE)

Apoptosis,Cancer

Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2.

P42575

Optimal dilutions for each application to be determined by the researcher.