alpha-Actinin-4 Antibody

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ProSci
Product Code: PSI-36-344
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-36-344-50ug50ug£898.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
F-actin Cross-linking Protein
Application Note:
Immunocytochemistry: (1:250- 1:1,600) . Immunohistochemistry: (1:250- 1:1,600) . Immunoprecipitation: (1:10) . Western Blot: (1:1,000-1:2,500) . Optimal conditions must be determined individually for each application.
Background:
alpha-Actinin 4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).
Buffer:
Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.
Concentration:
250 ug/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.
NCBI Gene ID #:
81
NCBI Official Name:
actinin, alpha 4
NCBI Official Symbol:
ACTN4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
12025678
Protein GI Number:
NP_004915
Purification:
Antigen affinity purified.
Research Area:
Cancer,Immunology
SPECIFICITY:
Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.
Swissprot #:
O43707
User NOte:
Optimal dilutions for each application to be determined by the researcher.