Apaf-1 Antibody [2E12]

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ProSci
Product Code: PSI-36-175
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-175-100ug100ug£609.00
Quantity:
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Overview

Host Type: Rat
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2E12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Apoptosis Protease-activating Factor 1
Application Note:
Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
Background:
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
NCBI Gene ID #:
317
NCBI Official Name:
apoptotic peptidase activating factor 1
NCBI Official Symbol:
APAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
2330015
Protein GI Number:
AAC51678
Purification:
>95% (SDS-PAGE)
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Swissprot #:
O14727
User NOte:
Optimal dilutions for each application to be determined by the researcher.