Fas Antibody [APO-1-3] (preservative free)

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ProSci
Product Code: PSI-36-167
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-167-50ug50ug£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG3
Antibody Clonality: Monoclonal
Antibody Clone: APO-1-3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS
Application Note:
ELISA: (direct or indirect: 1:2,000-1:10,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Background:
Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappaB.
Buffer:
Liquid. In PBS. Preservative free formulation.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human Fas.
NCBI Gene ID #:
355
NCBI Official Name:
Fas cell surface death receptor
NCBI Official Symbol:
FAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
4507583
Protein GI Number:
NP_000034.1
Purification:
>95% (SDS-PAGE)
Research Area:
Apoptosis,Cancer,Functional Assays
SPECIFICITY:
Recognizes human Fas.
Swissprot #:
P25445
User NOte:
Optimal dilutions for each application to be determined by the researcher.