DLK1 Antibody [PF299-1]

ProSci
Product Code: PSI-36-071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-071-100ug100ug£597.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PF299-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Further Information

Additional Names:
Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Application Note:
ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:100-1:500). Immunohistochemistry: (paraffin sections (1:100-1:500)). Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Background:
DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human DLK1 (extracellular domain).
NCBI Gene ID #:
8788
NCBI Official Name:
delta-like 1 homolog (Drosophila)
NCBI Official Symbol:
DLK1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Protein Accession #:
74136023
Protein GI Number:
NP_003827
Research Area:
Cancer
SPECIFICITY:
Recognizes human DLK1. Does not cross-react with mouse DLK1.
Swissprot #:
P80370
User NOte:
Optimal dilutions for each application to be determined by the researcher.