IDO Antibody

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ProSci
Product Code: PSI-36-228
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-228-100ug100ug£597.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Indoleamine 2,3-dioxygenase; INDO
Application Note:
ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:200-1:500). Immunocytochemistry: (1:500) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human IDO.
NCBI Gene ID #:
3620
NCBI Official Name:
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol:
IDO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
4504577
Protein GI Number:
NP_002155.1
Research Area:
Cancer,Immunology,Innate Immunity,Obesity
SPECIFICITY:
Recognizes human IDO. Detects a band of ~45kDa by Western blot.
Swissprot #:
P14902
User NOte:
Optimal dilutions for each application to be determined by the researcher.